A naturally occurring deletion mutant of figwort mosaic virus (caulimovirus) is generated by RNA splicing
Identifieur interne : 004979 ( Main/Exploration ); précédent : 004978; suivant : 004980A naturally occurring deletion mutant of figwort mosaic virus (caulimovirus) is generated by RNA splicing
Auteurs : Herman B. Scholthof [États-Unis] ; Fang C. Wu [États-Unis] ; Richard D. Richins [États-Unis] ; Robert J. Shepherd [États-Unis]Source :
- Virology [ 0042-6822 ] ; 1991.
English descriptors
- Teeft :
- Academic press, Base pairing, Branch site, Camv, Cauliflower, Cauliflower mosaic virus, Caulimovirus, Caulimovirus group, Caulimoviruses, Clone, Coat protein gene, Codon, Deletion, Encapsidated, Figwort, Figwort mosaic virus, Fmva, Fragment, Gene, Gene expression, Genome, Gowda, Inoculated, Insertion, Intron, Lariat formation, Mosaic, Mutant, Nucleotide, Pfhs, Pfmvsc3, Plant introns, Plant pathology, Plasmid, Protoplast, Pstl, Pstl fragment, Redundant plasmid, Replication, Richins, Sacl, Sall, Sall fragment, Sall site, Scholthof, Sequencing, Shepherd, Splice, Splice site, Splice sites, Stramonium, Transcriptase gene, Transcription, Unpublished observations, Unpublished results, Viral, Viral genome, Wild type, Xhol, Xhol linker, Xhol site.
Abstract
Abstract: A naturally occurring deletion mutant is observed in plants infected with figwort mosaic virus (FMV), a caulimovirus. The encapsidated mutant genome is formed spontaneously in association with two different strains of FMV in four host plant species. The mutant also appears when cloned wild-type viral DNA is used as the inoculum. The deletion mutant alone is not infectious and it appears unable to replicate after its formation, even in the presence of wild-type virus. The gene for chloramphenicol acetyltransferase was inserted at different positions in the deletion mutant genome, and subsequent transient assays showed that gene expression of the mutant occurs despite the deletion. Sequence analyses of the mutant genome revealed a deletion of a 1237-bp segment encompassing a major portion of the coat protein gene and the 5′ end of the downstream reverse transcriptase gene. This deletion is associated with consensus signals for RNA splicing including the conserved 5′ and 3′ splice sites plus surrounding sequences, putative branch point(s) for lariat formation, and an extremely high adenosine content (41 %) of the removed fragment. This suggests that splicing of the FMV full-length transcript has occurred prior to reverse transcription and this accounts for the presence and accumulation of encapsidated DNAs with the same deletion.
Url:
DOI: 10.1016/0042-6822(91)90845-3
Affiliations:
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Scholthof</name><affiliation wicri:level="1"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Plant Pathology, University of Kentucky, Lexington, Kentucky 40546-0091</wicri:regionArea><wicri:noRegion>Kentucky 40546-0091</wicri:noRegion></affiliation></author><author><name sortKey="Wu, Fang C" sort="Wu, Fang C" uniqKey="Wu F" first="Fang C." last="Wu">Fang C. Wu</name><affiliation wicri:level="1"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Plant Pathology, University of Kentucky, Lexington, Kentucky 40546-0091</wicri:regionArea><wicri:noRegion>Kentucky 40546-0091</wicri:noRegion></affiliation></author><author><name sortKey="Richins, Richard D" sort="Richins, Richard D" uniqKey="Richins R" first="Richard D." last="Richins">Richard D. Richins</name><affiliation wicri:level="1"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Plant Pathology, University of Kentucky, Lexington, Kentucky 40546-0091</wicri:regionArea><wicri:noRegion>Kentucky 40546-0091</wicri:noRegion></affiliation></author><author><name sortKey="Shepherd, Robert J" sort="Shepherd, Robert J" uniqKey="Shepherd R" first="Robert J." last="Shepherd">Robert J. Shepherd</name><affiliation wicri:level="1"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Plant Pathology, University of Kentucky, Lexington, Kentucky 40546-0091</wicri:regionArea><wicri:noRegion>Kentucky 40546-0091</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j">Virology</title><title level="j" type="abbrev">YVIRO</title><idno type="ISSN">0042-6822</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1991">1991</date><biblScope unit="volume">184</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="290">290</biblScope><biblScope unit="page" to="298">298</biblScope></imprint><idno type="ISSN">0042-6822</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0042-6822</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Academic press</term><term>Base pairing</term><term>Branch site</term><term>Camv</term><term>Cauliflower</term><term>Cauliflower mosaic virus</term><term>Caulimovirus</term><term>Caulimovirus group</term><term>Caulimoviruses</term><term>Clone</term><term>Coat protein gene</term><term>Codon</term><term>Deletion</term><term>Encapsidated</term><term>Figwort</term><term>Figwort mosaic virus</term><term>Fmva</term><term>Fragment</term><term>Gene</term><term>Gene expression</term><term>Genome</term><term>Gowda</term><term>Inoculated</term><term>Insertion</term><term>Intron</term><term>Lariat formation</term><term>Mosaic</term><term>Mutant</term><term>Nucleotide</term><term>Pfhs</term><term>Pfmvsc3</term><term>Plant introns</term><term>Plant pathology</term><term>Plasmid</term><term>Protoplast</term><term>Pstl</term><term>Pstl fragment</term><term>Redundant plasmid</term><term>Replication</term><term>Richins</term><term>Sacl</term><term>Sall</term><term>Sall fragment</term><term>Sall site</term><term>Scholthof</term><term>Sequencing</term><term>Shepherd</term><term>Splice</term><term>Splice site</term><term>Splice sites</term><term>Stramonium</term><term>Transcriptase gene</term><term>Transcription</term><term>Unpublished observations</term><term>Unpublished results</term><term>Viral</term><term>Viral genome</term><term>Wild type</term><term>Xhol</term><term>Xhol linker</term><term>Xhol site</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: A naturally occurring deletion mutant is observed in plants infected with figwort mosaic virus (FMV), a caulimovirus. The encapsidated mutant genome is formed spontaneously in association with two different strains of FMV in four host plant species. The mutant also appears when cloned wild-type viral DNA is used as the inoculum. The deletion mutant alone is not infectious and it appears unable to replicate after its formation, even in the presence of wild-type virus. The gene for chloramphenicol acetyltransferase was inserted at different positions in the deletion mutant genome, and subsequent transient assays showed that gene expression of the mutant occurs despite the deletion. Sequence analyses of the mutant genome revealed a deletion of a 1237-bp segment encompassing a major portion of the coat protein gene and the 5′ end of the downstream reverse transcriptase gene. This deletion is associated with consensus signals for RNA splicing including the conserved 5′ and 3′ splice sites plus surrounding sequences, putative branch point(s) for lariat formation, and an extremely high adenosine content (41 %) of the removed fragment. This suggests that splicing of the FMV full-length transcript has occurred prior to reverse transcription and this accounts for the presence and accumulation of encapsidated DNAs with the same deletion.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country></list><tree><country name="États-Unis"><noRegion><name sortKey="Scholthof, Herman B" sort="Scholthof, Herman B" uniqKey="Scholthof H" first="Herman B." last="Scholthof">Herman B. Scholthof</name></noRegion><name sortKey="Richins, Richard D" sort="Richins, Richard D" uniqKey="Richins R" first="Richard D." last="Richins">Richard D. Richins</name><name sortKey="Shepherd, Robert J" sort="Shepherd, Robert J" uniqKey="Shepherd R" first="Robert J." last="Shepherd">Robert J. Shepherd</name><name sortKey="Wu, Fang C" sort="Wu, Fang C" uniqKey="Wu F" first="Fang C." last="Wu">Fang C. Wu</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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